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KMID : 0525720110160010033
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Journal of Chitin and Chitosan
2011 Volume.16 No. 1 p.33 ~ p.38
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Anti-cancer Effects of Different Molecular Weight Chitosan Oligosaccharides in Human Colon Cancer Cells
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Eom Tae-Kil
Kim Young-Sang
Cho Young-Sook
Kim Se-Kwon
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Abstract
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Here we report the anti-proliferative effect of three kinds of chitosan oligosaccharides (COSs) with relatively high molecular weight COS (HMWCOS, 5-10 kDa), medium molecular weight COS (MMWCOS, 3-5 kDa) and low molecular weight COS (LMWCOS, 1-3 kDa) in HT-29 human colon cancer cells. Among all COSs, LMWCOS exhibited the highest anti-roliferative effects in HT-29 cells. Furthermore, LMWCOS induced apoptosis in a dose-dependent manner on HT-29 cells. Treatment of cells with LMWCOS also induced the increase in caspase activity, PARP cleavage, and pro-apoptotic proteins and the decrease in anti-apoptotic proteins. In addition, inflammatory proteins were down-regulated by LMWCOS. These results indicated that the potential inhibitory effect of LMWCOS against growth of HT-29 cells might be associated with induction of apoptosis through p53 and NF-κB dependent pathway.
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KEYWORD
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chitosan oligosaccharide, apoptosis, colon cancer
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